This estimator helps with determining the reagents and sequencing runs that are needed to arrive at the desired coverage for your experiment. Perform the following steps to run the estimator:

1. Click the button for the type of application.
2. Select the application or product from the dropdown menu.
3. Enter the input parameters in the open fields. The gray fields are locked, indicating that the value cannot be changed for that application or product.
4. Select the instruments you want to perform the estimation for.
5. Click Submit.

The estimator writes a table containing the number of lanes or flow cells you need to use for the desired product and parameters. You can also download the results in a comma-separated values file, so you can share data or use the tables in Excel.

 

Additional Considerations

• Output is an estimation based on recommended cluster density and read length; real output will vary.
• For more information about estimating coverage estimates, see the technical note Estimating Sequencing Coverage.
• If you plan a targeted resequencing or enrichment experiment, make sure to read the technical note Optimizing Coverage for Targeted Resequencing.
• The estimator uses an estimate of clusters passing filter commonly found for balanced genomes (such as PhiX or the human genome). If you plan to sequence an unbalanced genome, you may have a lower number of clusters passing filter, and consequently a lower output per lane.
• Estimations for WGS, enrichment, and some panels use the coverage needed. Explain the coverage-based estimations
• Estimations for most panels use the minimum number of clusters needed. Explain the cluster-based estimations
• Estimations for TruSight Cancer uses a defined number of samples dor each instrument and sequencing kit. Explain the sample-based estimations